PDF Notes: Tema 10 La genética molecular II

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    What are the three phases of transcription in prokaryotes?

    Involves initiation, elongation, and termination.

    What initiates transcription in prokaryotes?

    Promoters are recognized by RNA polymerase.

    How does elongation occur in prokaryotic transcription?

    RNA polymerase adds ribonucleotides in 5'-3' direction.

    What signals termination in prokaryotic transcription?

    Specific sequences in the DNA template trigger RNA polymerase release.

    What distinguishes rho-dependent termination from rho-independent termination?

    Rho-dependent requires a specific factor to separate RNA from DNA.

    What is a key feature of prokaryotic RNA transcripts?

    They are primary transcripts ready for translation without processing.

    Where does transcription occur in eukaryotes?

    In the nucleus of the cell.

    What is the role of RNA polymerase II in eukaryotic transcription?

    Transcribes messenger RNA and other non-ribosomal RNAs.

    What is the TATA box?

    A common promoter element located 25 nucleotides upstream.

    What happens during the elongation phase in eukaryotic transcription?

    RNA polymerase phosphorylates and synthesizes RNA in 5'-3' direction.

    What is splicing in eukaryotic transcription?

    The removal of introns and joining of exons in RNA.

    What is the function of the 5' cap in eukaryotic RNA?

    Protects RNA from degradation and aids in translation.

    What is reverse transcription?

    The process where RNA synthesizes DNA using RNA as a template.

    What is the role of ribosomes in translation?

    They synthesize proteins by linking amino acids.

    What does the genetic code do?

    Converts nucleotide sequences into amino acid sequences.

    What is a codon?

    A sequence of three nucleotides that codes for an amino acid.

    How is the genetic code characterized?

    It is universal, non-variable, and degenerative.

    What is the role of tRNA in translation?

    Decodes the genetic code and carries amino acids.

    What occurs during the initiation phase of translation?

    The small ribosomal subunit binds to mRNA and initiator tRNA.

    What happens during the elongation phase of translation?

    Amino acids are added to the growing polypeptide chain.

    What signifies the termination of translation?

    Release factors recognize stop codons and release the protein.