Master this deck with 20 terms through effective study methods.
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Involves culturing, transferring Tg, injecting into blastocysts, and implantation.
Allows for in vitro selection of transgenic cell lines.
Requires a selection gene in the transgene that must be eliminated later.
Pronuclear transfer directly modifies zygotes, while ES cells involve multiple steps.
Sperm are incubated with DNA to create transgenic animals.
The transgene may become non-functional or rearranged.
Recognized advancements in targeted gene manipulation techniques.
Involves exchange between homologous DNA sequences.
Long homologous sequences and a rare event occurrence.
Contains the gene of interest and homologous sequences for recombination.
It serves as a replacement or insertion vector for gene modification.
Larger homologous fragments increase the frequency of recombination.
Type Ω involves sequence replacement, while type O involves sequence insertion.
To enrich for recombinant ES cells by eliminating non-targeted integrations.
It can lead to deletion, insertion, or inversion of DNA segments.
It provides a survival advantage to cells by conferring drug resistance.
It makes cells sensitive to a drug, thus eliminating them from the culture.
It enhances the efficiency of gene targeting by reducing genetic variability.
It results in the loss of protein expression from the targeted gene.
It recognizes LoxP sites to facilitate DNA recombination events.